50.14 Red cell folate (categories 3)

Red cell folate data available for STOP cohort only.

Over or below median (median level calculated in STOP) <14900 vs ≥14900.

50.14.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	178.181094	1	<0.0001	***
rcfolate_gp3	1.581702	1	0.2	ns
GA_samp	72.949708	1	<0.0001	***

hPL (μg/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	41.6216409	1	<0.0001	***
rcfolate_gp3	0.2280064	1	0.6	ns
GA_samp	1128.5376199	1	<0.0001	***

GH2 (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	423.138396	1	<0.0001	***
rcfolate_gp3	2.101071	1	0.1	ns
GA_samp	564.814742	1	<0.0001	***

Insulin (uU/ml)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp3	1	Inf	0.807	0.807	0.4	ns
GA_samp	1	Inf	0.589	0.589	0.4	ns

HOMA-IR

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp3	1	Inf	0.947	0.947	0.3	ns
GA_samp	1	Inf	0.448	0.448	0.5	ns

Glucose (mmol/L)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp3	1	Inf	4.497	4.497	0.03	*
GA_samp	1	Inf	0.987	0.987	0.3	ns

50.14.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)

Var	Effect	P
(Intercept)	14.79 (9.96, 21.95)	<0.0001
rcfolate_gp3≥14900	1.07 (0.96, 1.19)	0.21
GA_samp	1.15 (1.11, 1.19)	<0.0001

hPL (μg/ml)

Var	Effect	P
(Intercept)	1.99 (1.61, 2.45)	<0.0001
rcfolate_gp3≥14900	0.99 (0.93, 1.05)	0.63
GA_samp	1.35 (1.33, 1.37)	<0.0001

GH2 (ng/ml)

Var	Effect	P
(Intercept)	0.05 (0.04, 0.06)	<0.0001
rcfolate_gp3≥14900	0.94 (0.87, 1.02)	0.15
GA_samp	1.35 (1.32, 1.39)	<0.0001

Insulin (uU/ml)

Var	Effect	P
(Intercept)	8.31 (5.12, 13.46)	<0.0001
rcfolate_gp3≥14900	1.06 (0.93, 1.22)	0.37
GA_samp	1.02 (0.97, 1.06)	0.44

HOMA-IR

Var	Effect	P
(Intercept)	1.77 (1.07, 2.92)	0.03
rcfolate_gp3≥14900	1.07 (0.93, 1.23)	0.33
GA_samp	1.02 (0.97, 1.06)	0.50

Glucose (mmol/L)

Var	Effect	P
(Intercept)	4.85 (4.43, 5.32)	<0.0001
rcfolate_gp3≥14900	1.04 (1.00, 1.08)	0.03
GA_samp	1.00 (0.99, 1.00)	0.32

50.14.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

50.14.4 Estimated marginal means

Outcome	rcfolate_gp3	response	SE	df	lower.CL	upper.CL
PRL (ng/ml)	<14900	72.355	6.142	71.241	61.089	85.700
PRL (ng/ml)	≥14900	77.551	6.690	75.947	65.309	92.088
hPL (μg/ml)	<14900	62.766	2.027	66.301	58.847	66.946
hPL (μg/ml)	≥14900	61.863	2.073	76.512	57.870	66.131
GH2 (ng/ml)	<14900	1.490	0.059	82.604	1.377	1.612
GH2 (ng/ml)	≥14900	1.401	0.058	97.719	1.290	1.522
Insulin (uU/ml)	<14900	10.044	0.430	Inf	9.236	10.923
Insulin (uU/ml)	≥14900	10.679	0.565	Inf	9.628	11.845
HOMA-IR	<14900	2.098	0.094	Inf	1.922	2.290
HOMA-IR	≥14900	2.250	0.126	Inf	2.016	2.511
Glucose (mmol/L)	<14900	4.657	0.048	Inf	4.564	4.753
Glucose (mmol/L)	≥14900	4.844	0.074	Inf	4.700	4.992

50.14.5 Plots

50.14.6 Post-hoc comparisons

Outcome	Contrast	Ratio of Geometric means (95% CI)	P-value
PRL (ng/ml)	≥14900 / <14900	1.07 (0.96, 1.19)	0.2
hPL (μg/ml)	≥14900 / <14900	0.99 (0.93, 1.05)	0.6
GH2 (ng/ml)	≥14900 / <14900	0.94 (0.87, 1.02)	0.1
Insulin (uU/ml)	≥14900 / <14900	1.06 (0.93, 1.22)	0.4
HOMA-IR	≥14900 / <14900	1.07 (0.93, 1.23)	0.3
Glucose (mmol/L)	≥14900 / <14900	1.04 (1.00, 1.08)	0.03