50.14 Red cell folate (categories 3)

Red cell folate data available for STOP cohort only.


Over or below median (median level calculated in STOP) <14900 vs ≥14900.

50.14.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 178.181094 1 <0.0001 ***
rcfolate_gp3 1.581702 1 0.2 ns
GA_samp 72.949708 1 <0.0001 ***
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 41.6216409 1 <0.0001 ***
rcfolate_gp3 0.2280064 1 0.6 ns
GA_samp 1128.5376199 1 <0.0001 ***
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 423.138396 1 <0.0001 ***
rcfolate_gp3 2.101071 1 0.1 ns
GA_samp 564.814742 1 <0.0001 ***
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp3 1 Inf 0.807 0.807 0.4 ns
GA_samp 1 Inf 0.589 0.589 0.4 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp3 1 Inf 0.947 0.947 0.3 ns
GA_samp 1 Inf 0.448 0.448 0.5 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp3 1 Inf 4.497 4.497 0.03 *
GA_samp 1 Inf 0.987 0.987 0.3 ns

50.14.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 14.79 (9.96, 21.95) <0.0001
rcfolate_gp3≥14900 1.07 (0.96, 1.19) 0.21
GA_samp 1.15 (1.11, 1.19) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.99 (1.61, 2.45) <0.0001
rcfolate_gp3≥14900 0.99 (0.93, 1.05) 0.63
GA_samp 1.35 (1.33, 1.37) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.05 (0.04, 0.06) <0.0001
rcfolate_gp3≥14900 0.94 (0.87, 1.02) 0.15
GA_samp 1.35 (1.32, 1.39) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 8.31 (5.12, 13.46) <0.0001
rcfolate_gp3≥14900 1.06 (0.93, 1.22) 0.37
GA_samp 1.02 (0.97, 1.06) 0.44
HOMA-IR
Var Effect P
(Intercept) 1.77 (1.07, 2.92) 0.03
rcfolate_gp3≥14900 1.07 (0.93, 1.23) 0.33
GA_samp 1.02 (0.97, 1.06) 0.50
Glucose (mmol/L)
Var Effect P
(Intercept) 4.85 (4.43, 5.32) <0.0001
rcfolate_gp3≥14900 1.04 (1.00, 1.08) 0.03
GA_samp 1.00 (0.99, 1.00) 0.32

50.14.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

50.14.4 Estimated marginal means

Outcome rcfolate_gp3 response SE df lower.CL upper.CL
PRL (ng/ml) <14900 72.355 6.142 71.241 61.089 85.700
PRL (ng/ml) ≥14900 77.551 6.690 75.947 65.309 92.088
hPL (μg/ml) <14900 62.766 2.027 66.301 58.847 66.946
hPL (μg/ml) ≥14900 61.863 2.073 76.512 57.870 66.131
GH2 (ng/ml) <14900 1.490 0.059 82.604 1.377 1.612
GH2 (ng/ml) ≥14900 1.401 0.058 97.719 1.290 1.522
Insulin (uU/ml) <14900 10.044 0.430 Inf 9.236 10.923
Insulin (uU/ml) ≥14900 10.679 0.565 Inf 9.628 11.845
HOMA-IR <14900 2.098 0.094 Inf 1.922 2.290
HOMA-IR ≥14900 2.250 0.126 Inf 2.016 2.511
Glucose (mmol/L) <14900 4.657 0.048 Inf 4.564 4.753
Glucose (mmol/L) ≥14900 4.844 0.074 Inf 4.700 4.992

50.14.5 Plots

50.14.6 Post-hoc comparisons

Outcome Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) ≥14900 / <14900 1.07 (0.96, 1.19) 0.2
hPL (μg/ml) ≥14900 / <14900 0.99 (0.93, 1.05) 0.6
GH2 (ng/ml) ≥14900 / <14900 0.94 (0.87, 1.02) 0.1
Insulin (uU/ml) ≥14900 / <14900 1.06 (0.93, 1.22) 0.4
HOMA-IR ≥14900 / <14900 1.07 (0.93, 1.23) 0.3
Glucose (mmol/L) ≥14900 / <14900 1.04 (1.00, 1.08) 0.03